Imaging

Imaging Innate Immune Cells

Movie 1. Whole tissue imaging of Ly6G+ neutrophils and glomeruli in infected kidneys. Ly6g cre IVM (Hasenberg, 2015, Nat Methods) were infected with uropathogenic E.coli. One day later,  APC-conjugated CD31 antibody were administered intravenously to image neutrophils (red) and the vasculature / renal glomeruli (blue). Whole tissue imaging were performed by light-sheet microscopy.

Movie 2. Imaging of blood vessels in the urinary bladder. C57Bl/6 animals were injected with APC-conjugated CD31 antibody to image the vasculature. Whole tissue imaging were performed by light-sheet microscopy.

Movie 3. In vivo imaging of neutrophils and macrophages. Ly6G+ Neutrophils (red) and CX3CR1+ macrophages (green) communicate to phagocytose Debris. Movies were recorded in Ly6g cre IVM (Hasenberg, 2015, Nat Methods) x Cx3cr1 GFP (Jung, 2000, Mol Cell Biol) animals.

Movie 4. Spatial distribution of neutrophils and macrophages. The distribution of Ly6G+ Neutrophils (red) and CX3CR1+ macrophages (green) were analyzed in Ly6g cre IVM (Hasenberg, 2015, Nat Methods) x Cx3cr1 GFP (Jung, 2000, Mol Cell Biol) animals. Whole tissue imaging were performed by light-sheet microscopy.

Bildschirmfoto 2016-02-24 um 22.07.41 Kopie

Neutrophil-mapping1
Bildschirmfoto 2016-05-19 um 20.58.12 Kopie