Local inflammation around the renal glomerulus

Leukocyte Migration

Glomerulus in pink, Nuclei in blue, macrophages in green, neutrophils in red.

Schiwon et al. Cell 2014

Communication

Cross talk of two types of macrophages regulates neutrophil migration into the infected epithelium.

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Chemokine secretion

Smart & Simple

Secretion of prestored chemokines by tissue macrophages.

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Bad bugs

Infection

Pathogenic E.coli infection invade into mucosal epithelium

Transepithelial migration

Neutrophils

Monocyte-derived Tumor Necrosis Factor is the fundamental licensing factor

Sophisticated

Flow cytometry

Cell sorting, a prerequisite for subsequent cellular analyses

Cover on the Journal of Immunology

Macrophages

Local Proliferation of macrophages.

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Landscape Immunology - High dimensional proteome analysis

The immune system integrates information across multiple scales to develop an effective response to invading pathogens and to regulate tissue specific immunity. Modern approaches in high dimensional proteome microscopy are key to understanding the complexities of this spatial regulation. We use multimodal imaging approaches by combining microscopy with mass spectrometry to analyse the local regulation of immune responses on histological sections. The interdisciplinary analysis combines immunohistochemistry (IHC), matrix-assisted laser desorption/ ionization imaging mass spectrometry (MALDI IMS) and liquid chromatography with tandem mass spectrometry (LC-MS/MS) to unravel novel mechanisms that spatially regulate tissue specific immunity.

Crosstalk of tissue macrophages and neutrophils 

Neutrophils are continuously generated within the bone marrow and released in the circulation. They rapidly migrate into infected tissues and significantly contribute to the inflammatory and antimicrobial process. However, the mechanisms that regulate the interstitial migration and the function of neutrophils in infected organs are incompletely understood. We use state of the imaging techniques to analyse the role of tissue macrophages, which form a dense network of sentinel cells within tissues, in regulating neutrophil migration and function in inflamed tissues.  

 

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Macrophages

Resident and inflammatory macrophages
Regulation of Immunology
Induction of immunity

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Neutrophils

Recruitment from the circulation
Interstitial migration
Crossing of barriers in Immunology

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Immunology

Cutting edge microscopy
Computational analysis in Immunology Proteomics and MALDI Imaging