Local inflammation around the renal glomerulus

Leukocyte Migration

Glomerulus in pink, Nuclei in blue, macrophages in green, neutrophils in red.

Schiwon et al. Cell 2014

Communication

Cross talk of two types of macrophages regulates neutrophil migration into the infected epithelium.

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Chemokine secretion

Smart & Simple

Secretion of prestored chemokines by tissue macrophages.

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Bad bugs

Infection

Pathogenic E.coli infection invade into mucosal epithelium

Transepithelial migration

Neutrophils

Monocyte-derived Tumor Necrosis Factor is the fundamental licensing factor

Sophisticated

Flow cytometry

Cell sorting, a prerequisite for subsequent cellular analyses

Cover on the Journal of Immunology

Macrophages

Local Proliferation of macrophages.

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Research focus

Monocytes, Macrophages and neutrophils play an important role in host defense against invading microorganisms. The tremendous migratory and functional capabilities of these innate leukocytes are crucial in initiating inflammatory processes, but also for maintaining organ homeostasis. However, the mechanisms that regulate the migration and function of these cells are incompletely understood. Our aim is to unravel the mechanisms that regulate the migration, the function and the cellular crosstalk between these innate immune cells. These findings are important to develop novel and selective targeting strategies to regulate immune responses.

Cellular crosstalk of macrophages and neutrophils

Neutrophils are continuously generated within the bone marrow by myeloid precursors and their half-life is limited to a few hours. Their main function is to patrol vessels and vigilantly seeking out for indications of an incipient inflammation. Recruitment of neutrophils is induced by stimuli, such as chemokines, leukotrienes and formyl peptides. Interstitial migration seems to be facilitated by integrins, actin polymerisation, chemokine receptors, TREM-1 expression and Matrix Metalloproteinases. Once recruited from the bloodstream, neutrophils cross the dense macrophage network and acquire essential properties for optimal anti-microbial defence. Our aim in to understand neutrophil migration under inflammatory conditions.

 

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Macrophages

Resident and inflammatory macrophages
Regulation of homeostasis
Induction of immunity

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Neutrophil migration

Recruitment from the circulation
Interstitial migration
Crossing of barriers

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Techniques

Cutting edge microscopy
Flow cytometry and cell sorting
"Omic" approches and epigenetics