Local inflammation around the renal glomerulus

Leukocyte Migration

Glomerulus in pink, Nuclei in blue, macrophages in green, neutrophils in red.

Schiwon et al. Cell 2014


Cross talk of two types of macrophages regulates neutrophil migration into the infected epithelium.

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Chemokine secretion

Smart & Simple

Secretion of prestored chemokines by tissue macrophages.

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Bad bugs


Pathogenic E.coli infection invade into mucosal epithelium

Transepithelial migration


Monocyte-derived Tumor Necrosis Factor is the fundamental licensing factor


Flow cytometry

Cell sorting, a prerequisite for subsequent cellular analyses

SPRING: Spatial PRoteome ImagiNG

Bioinformatics and pathway analysis

Multimodal mass spectrometry imaging combined annotation, visualization and integrated discovery research.

Spatial proteomics, metabolomics, lipidomics

The immune system integrates critical information across multiple scales to develop an effective response against invading pathogens and cancer. Modern approaches, such as imaging mass spectrometry and state-of-the-art microscopy, are key technologies to understand the mechanisms that spatially regulate leukocyte function. Multimodal imaging approaches, such as microscopy and mass spectrometry, are capable to analyze the local regulation of immune responses in infections and cancer. Our interdisciplinary coregistration algorithm combines immunohistochemistry (IHC), matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) and liquid chromatography with tandem mass spectrometry (LC-MS/MS) to unravel the immunological landscapes in diseases to improve diagnosis and therapies. 

Crosstalk of tissue macrophages and neutrophils 

Neutrophils are continuously generated within the bone marrow and released in the circulation. They rapidly migrate into infected tissues and significantly contribute to the inflammatory and antimicrobial process. However, the mechanisms that regulate the interstitial migration and the function of neutrophils in infected organs are incompletely understood. We use state of the imaging techniques to analyse the role of tissue macrophages, which form a dense network of sentinel cells within tissues, for the  regulation of neutrophil migration and function in inflamed tissues.  




Resident and inflammatory macrophages
Regulation of Immunology
Induction of immunity



Recruitment from the circulation
Interstitial migration
Crossing of barriers in Immunology



Cutting edge microscopy
Computational analysis in Immunology Proteomics and MALDI Imaging