Local inflammation around the renal glomerulus

Leukocyte Migration

Glomerulus in pink, Nuclei in blue, macrophages in green, neutrophils in red.

Schiwon et al. Cell 2014

Communication

Cross talk of two types of macrophages regulates neutrophil migration into the infected epithelium.

Read More

Chemokine secretion

Smart & Simple

Secretion of prestored chemokines by tissue macrophages.

Read More

Bad bugs

Infection

Pathogenic E.coli infection invade into mucosal epithelium

Transepithelial migration

Neutrophils

Monocyte-derived Tumor Necrosis Factor is the fundamental licensing factor

Sophisticated

Flow cytometry

Cell sorting, a prerequisite for subsequent cellular analyses

Cover on the Journal of Immunology

Macrophages

Local Proliferation of macrophages.

Read More

Landscape proteomics, metabolomics, lipidomics

The immune system integrates critical information across multiple scales to develop an effective response against invading pathogens and cancer. Modern approaches, such as imaging mass spectrometry and state-of-the-art microscopy are key technologies to understand the mechanisms that spatially regulate leukocyte function. We use multimodal imaging approaches by combining microscopy and mass spectrometry to analyse the local regulation of immune responses in infections and cancer. Our interdisciplinary coregistration algorithm combines immunohistochemistry (IHC), matrix-assisted laser desorption/ ionization mass spectrometry imaging (MALDI MSI) and liquid chromatography with tandem mass spectrometry (LC-MS/MS) to improve diagnosis and therapies. 

Crosstalk of tissue macrophages and neutrophils 

Neutrophils are continuously generated within the bone marrow and released in the circulation. They rapidly migrate into infected tissues and significantly contribute to the inflammatory and antimicrobial process. However, the mechanisms that regulate the interstitial migration and the function of neutrophils in infected organs are incompletely understood. We use state of the imaging techniques to analyse the role of tissue macrophages, which form a dense network of sentinel cells within tissues, for the  regulation of neutrophil migration and function in inflamed tissues.  

 

box1

Macrophages

Resident and inflammatory macrophages
Regulation of Immunology
Induction of immunity

box2

Neutrophils

Recruitment from the circulation
Interstitial migration
Crossing of barriers in Immunology

box3

Immunology

Cutting edge microscopy
Computational analysis in Immunology Proteomics and MALDI Imaging